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In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Gene therapy --- Gene Transfer Techniques --- Genetic Vectors --- Genetic Techniques --- Genetic Structures --- Genetic Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Pathology --- Medicine --- Health & Biological Sciences --- Methodology --- Genetic Therapy --- Thérapie génique --- methods. --- Methodology. --- Méthodologie --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Therapy, Gene --- methods --- Human genetics. --- Animal genetics. --- Gene therapy. --- Human Genetics. --- Animal Genetics and Genomics. --- Gene Therapy. --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology

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Health services administration --- Epidemiology --- Public Health --- Health Care --- Health Occupations --- Investigative Techniques --- Environment and Public Health --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Disciplines and Occupations --- Epidemiologic Methods --- Health Services Administration --- Health & Biological Sciences --- Hospitals & Medical Centers --- Community-Based Distribution --- Contraceptive Distribution --- Delivery of Healthcare --- Dental Care Delivery --- Distribution, Non-Clinical --- Distribution, Nonclinical --- Distributional Activities --- Healthcare --- Healthcare Delivery --- Healthcare Systems --- Non-Clinical Distribution --- Nonclinical Distribution --- Delivery of Dental Care --- Health Care Delivery --- Health Care Systems --- Activities, Distributional --- Activity, Distributional --- Care, Health --- Community Based Distribution --- Community-Based Distributions --- Contraceptive Distributions --- Deliveries, Healthcare --- Delivery, Dental Care --- Delivery, Health Care --- Delivery, Healthcare --- Distribution, Community-Based --- Distribution, Contraceptive --- Distribution, Non Clinical --- Distributional Activity --- Distributions, Community-Based --- Distributions, Contraceptive --- Distributions, Non-Clinical --- Distributions, Nonclinical --- Health Care System --- Healthcare Deliveries --- Healthcare System --- Non Clinical Distribution --- Non-Clinical Distributions --- Nonclinical Distributions --- System, Health Care --- System, Healthcare --- Systems, Health Care --- Systems, Healthcare --- Community Health --- Environment, Preventive Medicine & Public Health --- Environment, Preventive Medicine and Public Health --- Health, Community --- Health, Public --- Preventive Medicine --- Education, Public Health Professional --- Diseases --- Public health --- Health administration --- Health care administration --- Health care management --- Health sciences administration --- Health services management --- Medical care --- Health planning --- Public health administration --- Administration, Health Services --- Health Services --- Epidemiologic Method --- Epidemiological Methods --- Methods, Epidemiologic --- Epidemiological Method --- Method, Epidemiologic --- Method, Epidemiological --- Methods, Epidemiological --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Health Professions --- Health Occupation --- Health Profession --- Profession, Health --- Professions, Health --- Occupations --- Methodology --- Administration --- Management --- organization & administration --- methods

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Cataract. --- Cataracte (Ophtalmologie) --- Cataract --- Refractive Surgical Procedures --- Lens Diseases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Investigative Techniques --- Ophthalmologic Surgical Procedures --- Eye Diseases --- Surgical Procedures, Operative --- Diseases --- Cataract Extraction --- Diagnosis --- Methods --- Medicine --- Health & Biological Sciences --- Ophthalmology & Optometry --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Cataract, Membranous --- Lens Opacities --- Pseudoaphakia --- Cataracts --- Cataracts, Membranous --- Lens Opacity --- Membranous Cataract --- Membranous Cataracts --- Opacities, Lens --- Opacity, Lens --- Pseudoaphakias --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- Phakectomy --- Enzymatic Zonulolysis --- Cataract Extractions --- Enzymatic Zonulolyses --- Extraction, Cataract --- Extractions, Cataract --- Phakectomies --- Zonulolyses, Enzymatic --- Zonulolysis, Enzymatic --- Ghost Surgery --- Operative Procedures --- Operative Surgical Procedure --- Operative Surgical Procedures --- Procedure, Operative Surgical --- Procedures, Operative Surgical --- Surgery, Ghost --- Surgical Procedure, Operative --- Operative Procedure --- Procedure, Operative --- Procedures, Operative --- General Surgery --- Eye Disorders --- Eye Disease --- Eye Disorder --- Ophthalmology --- Ophthalmologic Surgical Procedure --- Ophthalmological Surgical Procedures --- Procedure, Ophthalmologic Surgical --- Procedures, Ophthalmologic Surgical --- Surgical Procedure, Ophthalmologic --- Surgical Procedures, Ophthalmologic --- Ophthalmological Surgical Procedure --- Procedure, Ophthalmological Surgical --- Procedures, Ophthalmological Surgical --- Surgical Procedure, Ophthalmological --- Surgical Procedures, Ophthalmological --- Eye --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Disease, Lens --- Diseases, Lens --- Lens Disease --- Refractive Surgery --- Keratorefractive Surgical Procedures --- Keratorefractive Surgical Procedure --- Procedure, Keratorefractive Surgical --- Procedure, Refractive Surgical --- Procedures, Keratorefractive Surgical --- Procedures, Refractive Surgical --- Refractive Surgeries --- Refractive Surgical Procedure --- Surgeries, Refractive --- Surgery, Refractive --- Surgical Procedure, Keratorefractive --- Surgical Procedure, Refractive --- Surgical Procedures, Keratorefractive --- Surgical Procedures, Refractive --- Refractive Errors --- Orthokeratologic Procedures --- Crystalline lens --- diagnosis --- surgery --- Diagnose --- Surgical Procedures --- Procedure, Surgical --- Procedures, Surgical --- Surgical Procedure --- Techniques --- Technique

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Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. This volume thus discusses the isolation and functional characterization of cells involved in innate immunity in mouse and man, including mast cells and eosinophils. Other focuses include natural killer cells, methods in statistics, in vivo imaging, genome engineering, and mutagenesis and culture that are adapted to the study of innate immunity in these hosts. These are complemented with a series of chapters dealing with alternative models: plants, worms, mosquitoes, flies, and fish. Together, these approaches and models are being used to dissect the complex interplay between hosts and pathogens and contribute to developing strategies to help fight infection. With chapters written by experts on the cutting-edge of this technology, Innate Immunity is an essential reference for immunologists, histologists, geneticists, and molecular biologists.

Natural immunity --- Immunology, Comparative --- Killer Cells, Natural --- Laboratory Manuals --- Genetic Techniques --- Immunologic Techniques --- Cytological Techniques --- Immunity, Innate --- Lymphocytes --- Publication Formats --- Clinical Laboratory Techniques --- Immunity --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Immune System Phenomena --- Leukocytes, Mononuclear --- Publication Characteristics --- Leukocytes --- Phenomena and Processes --- Blood Cells --- Immune System --- Cells --- Hemic and Immune Systems --- Blood --- Anatomy --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- Natural immunity. --- Immunology, Comparative. --- Immunité naturelle --- Immunologie comparée --- EPUB-LIV-FT LIVBIOMO LIVMEDEC SPRINGER-B --- White Blood Corpuscles --- Blood Cells, White --- Blood Corpuscles, White --- White Blood Cells --- Blood Cell, White --- Blood Corpuscle, White --- Corpuscle, White Blood --- Corpuscles, White Blood --- Leukocyte --- White Blood Cell --- White Blood Corpuscle --- Mononuclear Leukocytes --- Leukocyte, Mononuclear --- Mononuclear Leukocyte --- Immune System Concepts --- Immune System Phenomenon --- Immune System Process --- Immune System Processes --- Concept, Immune System --- Concepts, Immune System --- Immune System Concept --- Phenomena, Immune System --- Phenomenon, Immune System --- Process, Immune System --- Processes, Immune System --- Anatomies --- Water --- Hemic System --- System, Hemic --- Cell --- Immune Systems --- System, Immune --- Systems, Immune --- Blood Corpuscles --- Blood Cell --- Blood Corpuscle --- Cell, Blood --- Cells, Blood --- Corpuscle, Blood --- Corpuscles, Blood --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Disease --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Lymphoid Cells --- Cell, Lymphoid --- Cells, Lymphoid --- Lymphocyte --- Lymphoid Cell --- Innate Immune Response --- Innate Immunity --- Immunity, Native --- Immunity, Natural --- Immunity, Non-Specific --- Resistance, Natural --- Immune Response, Innate --- Immune Responses, Innate --- Immunity, Non Specific --- Innate Immune Responses --- Native Immunity --- Natural Immunity --- Natural Resistance --- Non-Specific Immunity --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- NK Cells --- Natural Killer Cells --- Cell, NK --- Cell, Natural Killer --- Cells, NK --- Cells, Natural Killer --- Killer Cell, Natural --- NK Cell --- Natural Killer Cell --- Comparative immunology --- Disease resistance --- Host resistance --- Innate immunity --- Innate resistance --- Native immunity --- Natural resistance --- Nonspecific immunity --- Resistance to disease --- blood --- cytology --- immunology --- methods --- Immunology. --- Cytology. --- Human genetics. --- Medicine. --- Cell Biology. --- Human Genetics. --- Molecular Medicine. --- Cell biology --- Cellular biology --- Cytologists --- Immunobiology --- Life sciences --- Serology --- Heredity, Human --- Human biology --- Physical anthropology --- Health Workforce

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Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.

Computational Biology --- Computational biology. --- Genes, Essential --- Genetic Techniques --- Genetics, Microbial --- Genome, Bacterial --- Genomics --- Microbial genetics. --- Microbiological Techniques --- genetics --- methods --- Microbial genetics --- Computational biology --- Genetics --- Laboratory Manuals --- Methods --- Investigative Techniques --- Genome --- Biology --- Clinical Laboratory Techniques --- Microbiology --- Publication Formats --- Genes --- Genetic Structures --- Genome Components --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Natural Science Disciplines --- Genetic Phenomena --- Disciplines and Occupations --- Phenomena and Processes --- Health & Biological Sciences --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Component, Genome --- Components, Genome --- Genome Component --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Cistron --- Gene --- Genetic Materials --- Cistrons --- Genetic Material --- Material, Genetic --- Materials, Genetic --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Genomes --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Microbiologic Technic --- Microbiologic Technics --- Microbiologic Technique --- Microbiological Technics --- Technic, Microbiologic --- Technics, Microbiological --- Technique, Microbiologic --- Techniques, Microbiologic --- Microbiologic Techniques --- Microbiological Technic --- Microbiological Technique --- Technic, Microbiological --- Technics, Microbiologic --- Technique, Microbiological --- Techniques, Microbiological --- Bio-Informatics --- Biology, Computational --- Computational Molecular Biology --- Bioinformatics --- Molecular Biology, Computational --- Bio Informatics --- Bio-Informatic --- Bioinformatic --- Biologies, Computational Molecular --- Biology, Computational Molecular --- Computational Molecular Biologies --- Molecular Biologies, Computational --- Microbial Genetics --- Genetic, Microbial --- Microbial Genetic --- Bacterial Genomes --- Genomes, Bacterial --- Bacterial Genome --- Microorganisms --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genes, House-keeping --- Genes, Housekeeping --- Essential Gene --- Essential Genes --- Gene, Essential --- Gene, House-keeping --- Gene, Housekeeping --- Genes, House keeping --- House-keeping Gene --- House-keeping Genes --- Housekeeping Gene --- Housekeeping Genes --- Biochemistry. --- Cytology. --- Human genetics. --- Biochemistry, general. --- Cell Biology. --- Human Genetics. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Heredity, Human --- Human biology --- Physical anthropology --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Composition